Fully-Automated Medium and Reagent Pipetting System for Live Cell Imaging

1. It meets the standard ANSI/SBS footprint and fits in the Stage Top's Incubator. You can easily set up various protocols on your PC, and the system will automatically conduct the pipetting processes you currently carry out manually. The pump heads and other wetted parts are disposable.
2. Easily programmable from rapid medium replace to gentle perfusion. Researchers can press "start" to begin fully-automatic pipetting and focus entirely on the observation of cells.
3. Capable of slow and precise reagent addition (μL/min level), which is difficult with conventional manual pipetting. A controlled supply speed results in stable images.
   

   *: A certain level of pulsation appears when peristaltic pumps are being used.

4. Gradient mode can control concentration by gradually changing the flow rates of the 2 perfusion pumps. (Only available for CEIM-0200 Series)
5. Maximum of 4 types of reagent addition pump are available with different flow rates. Please get in touch with us for more information or any questions about this fluidic system.

Note: Details such as specifications, etc., may be changed without notice.

APPLICATION EXAMPLE

As the dosing action doesn't disturb imaging, even though using with high powered / high NA microscope (60X, NA1.35), it is possible to clearly observe the images of before/after dosing the reagent.

1. Feed the HeLa cells into the glass-bottom dish, and transfect the genes.
2. After incubating for one day, add 1ml of reagent to stimulate the genes while photographing.
3. The change in fluorescence wavelength of GEM-GECO1 inside the cells (when GEM-GECO1 is chemically bonded to calcium ion, the color of the fluorescence wavelength shifts from green to blue) was observed.

[EXPERIMENT CONDITIONS]

• Microscope: Inverted confocal laser microscope
• Magnification of objective lens: 60x
• The numerical aperture of objective lens: 1.35
• Cells: HeLa cells
• Reagent: ionomycin

Note 1: Details such as specifications, etc., may be changed without notice.

Note 2: Data courtesy of Dr. H. Shibata and Mr. T. Hayashimoto, Molecular and Cellular Regulation Lab., Graduate School of Bioagricultural Sciences, Nagoya University (Japan)

SYSTEM CONFIGURATION

VIDEO OF THE LIVE CELL IMAGING FLUIDIC SYSTEM

Note: Details such as specifications, etc., may be changed without notice.

SPECIFICATIONS

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